HPLC Columns

Reversed-Phase

PDF Hamilton reversed-phase HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting. Hamilton offers four polymeric and two silica-based packing materials for reversed-phase separations. PS-DVB resins are similar in retention characteristics to silica C18s in that retention tends to increase with lipophilicity. …

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Anion Exchange

PDF In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the …

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Cation Exchange

PDF In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the positive (+) charge on the sample, the stronger it will be attracted to the negative charge on …

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Ion Exclusion

PDF Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later. Mixtures of weak acids, like those in fruits and milk products, are frequently not very well separated by pure ion-exchange …

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